Description
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of a1AGP in porcine serum, plasma or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to a1AGP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to a1AGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain a1AGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of a1AGP in the samples is then determined by comparing the O.D. of the samples to the standard curve